Nerve Cell Culturing

Compartmented Culture Chambers & Tooling

Developed originally as a model system to study the formation and maintenance of neuronal projections, the Campenot Compartmented Culture System provides unique experimental capabilities for the analysis of neural cell function and physiology. In compartmented culture, axons (neurites) emanating from neurons cultured in a proximal compartment grow across a water-tight barrier and enter into a separate fluid environment within a distal compartment. This mimics the neuronal cell bodies in one location in the nervous system projecting their axons through nerves or tracts to targets in another location.

Tooling

Pin Rake

The pin rake (CAMP-PR) required for controlled scratching of the collagen/plastic (or polylysine/plastic) substratum to promote directed growth consists of a precisely aligned array of stainless steel pins in a ceramic holder. The device is used to produce parallel collagen tracks approximately 200 um wide.

Standard Configurations: CAMP-PR Pin Rake, 21 Pin Array with Protective Cap

Syringe Grease Applicator

The syringe grease applicator (CAMP GLSS) is used with a needle to apply sterile silicone grease to the compartmented chamber septa so that a water-tight seal is maintained between compartments. It is machined from medical grade stainless steel and has large handles to assist in extruding the highly viscous silicone grease.

Standard Configurations: CAMP-GLSS Stainless Steel Syringe with Luer-lok Tip

Chambers

Custom designed chambers are available. For most configurations a one-time programming charge of $85 is applied, with subsequent cost of production of chambers dependent upon material used and the time required to cut and polish.